Advances in high throughput LC/MS based metabolomics: A review

Properly implemented, metabolic and lipidomic profiling can provide a deeper understanding of mammalian, plant bacterial biology. These omics-tools have developed matured over the last 40-years are now being deployed to valuable information in epidemiological studies, drug toxicology pharmacology, disease biology progression patient stratification. LC/MS has become technology choice for both lipid profiling, due its speed, sensitivity structural elucidation capabilities. In preceding two decades there been many technological methodological advances that facilitated evolution into rugged, reliable, easily tool. include, but not limited to, improvements chromatography (phases, columns, delivery system), instruments mass spectrometry, optimization sample preparation, introduction ion mobility, data analysis tools, metabolite databases, harmonized protocols, more widespread use quality control methods reference standards/matrices. Here, recent developments high throughput liquid chromatography/high resolution spectrometry phenotyping described. which may improved feature detection, increased laboratory efficiency quality, as well “biomarker” identification, discussed relation their potential application large clinical or biobank collections.